Immunocytochemistry

ICC 1

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Hematoxylin and eosin (H&E)-stained section of a poorly differentiated tumor found in brain biopsy. The polygonal shape of the cells and their cohesion suggest an epithelial tumor (i.e. a carcinoma).

ICC 2

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Immunocytochemical study of same tumor as in slide 1 using monoclonal antibody to low molecular weight cytokeratin using antibody 35ßH11, showing a complete absence of reactivity.

ICC 3

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Immunocytochemical study of same tumor as in slide 1 using monoclonal antibody HMB45 to melanoma. Positive antibody binding is indicated by the black reaction product. This result indicates that the tumor is a variant of a melanoma, not a carcinoma.

ICC 4

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Summary of antibody studies useful in determining site of origin of carcinomas presenting at metastatic sites.

ICC 5

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Hematoxylin and eosin (H&E)-stained section of tumor in supraclavicular lymph node found in 72 year old male. It is not obvious where this poorly differentiated carcinoma metastasized from.

ICC 6

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Immunocytochemical study of same tumor cells as in slide 5 employing antibodies to prostatic specific antigen, showing positive reaction, and pointing to site of origin of tumor in prostate.