EDS type VIIA (COL1A1) and EDS type VIIB (COL1A2) gDNA – Molecular study without prior collagen screening study

CPT Code	Description	Price
83898	PCR amplification COL1A1 and COL1A2 exon 6 from genomic DNA (x2)
83894	Polyacrylamide gel electrophoresis (x1)
83904	Mutation identification by bidirectional sequencing (x4)
83912	Interpretation & Report
Total		$268

EDS type VIIA (COL1A1) gDNA – Molecular study after positive collagen study result

CPT Code	Description	Price
83891	RNA isolation from cultured cells
83891	DNA extraction from cultured cells
83902	Synthesis of cDNA (reverse transcription)
83898	PCR amplification COL1A1 exon 6 from cDNA
83898	PCR amplification COL1A1 exon 6 from genomic DNA
83894	Polyacrylamide gel electrophoresis (x1)
83904	Mutation identification by bidirectional sequencing of cDNA amplification product (x2)
83904	Mutation identification by bidirectional sequencing of genomic DNA amplification product (x2)
83912	Interpretation & Report
Total		$404

EDS type VIIB (COL1A2) gDNA – Molecular study after positive collagen study result

CPT Code	Description	Price
83891	RNA isolation from cultured cells
83891	DNA extraction from cultured cells
83902	Synthesis of cDNA (reverse transcription)
83898	PCR amplification COL1A2 exon 6 from cDNA
83898	PCR amplification COL1A2 exon 6 from genomic DNA
83894	Polyacrylamide gel electrophoresis (x1)
83904	Mutation identification by bidirectional sequencing of cDNA amplification product (x2)
83904	Mutation identification by bidirectional sequencing of genomic DNA amplification product (x2)
83912	Interpretation & Report
Total		$404