Services
.
We accept samples 24 hours / day, 7 days / week. Please notify the laboratory if you are sending a specimen. Weekdays at 206-598-4488. On weekends please have the Cytogenetics on-call faculty paged at 206-598-6190.
 
Neoplastic Bone Marrow or Blood Cytogenetic Analysis
This Analysis is for the detection of acquired chromosome anomalies in leukemia or lymphoma. It includes:
  • NUMBER OF G-BANDED CELLS EXAMINED: 20-30.
  • TYPE OF BANDING: G-banding routinely used.
  • BANDING LEVEL: variable, depending on type of neoplasia.
  • KARYOTYPES: 20 G-banded cells completely analyzed. A minimum of two karyotypes.
  • Interphase cell analysis by FISH. As an adjunct to Cytogenetics or as FISH only.List of probes here.

 

Amniotic Fluid Cytogenetic Analysis
This prenatal test (at about 15-16 weeks gestation) combines the examination of both clones and cells from a mass culture. This helps the interpretation of mosaic cases and provides the best banding resolution, respectively. The complete analysis includes:
  • NUMBER OF G-BANDED CELLS EXAMINED: 15-20.
  • TYPE OF BANDING: G-banding routinely used. R-, Q-, C-, NOR-, DA-DAPI- bandings as needed.
  • BANDING LEVEL: 400-550 bands.
  • KARYOTYPES: 6 G-banded cells completely analyzed. Two karyotypes. Panels of chromosomes as needed.
  • Interphase FISH for aneuploidy if requested.
NOTE: These cells can be grown for additional biochemical or genetic tests.
 
Chorionic Villi Cytogenetic Analysis
This prenatal test, which is carried out at about 10-11 week gestation, is based on a direct preparation for a preliminary diagnosis at 24 hours and a long-term culture for a definitive diagnosis. The complete analysis includes:
  • NUMBER OF G-BANDED CELLS EXAMINED: 20.
  • TYPE OF BANDING: G-banding routinely used. R-, Q-, C-, NOR-, DA-DAPI- bandings as needed.
  • BANDING LEVEL: 400-450 bands.
  • KARYOTYPES: 6 G-banded cells completely analyzed. Two karyotypes. Panels of chromosomes as needed.
  • Interphase FISH for aneuploidy if requested.

 

Solid Tissue Cytogenetic Analysis
This analysis, which is done on skin samples (e.g., to further evaluate a case of mosaicism), on abortus or autopsy material (e.g., in cases of spontaneous abortion or stillborn), includes:
  • NUMBER OF G-BANDED CELLS EXAMINED: 20.
  • TYPE OF BANDING: G-banding routinely used. R-, Q-, C-, NOR-, DA-DAPI- bandings as needed.
  • BANDING LEVEL: 400-550 bands.
  • KARYOTYPES: 5 G-banded cells completely analyzed. Two karyotypes. Panels of chromosomes as needed.
Tumor Cytogenetic Analysis
This analysis for the detection of acquired chromosome anomalies in sarcoma and other solid tumors includes:
  • NUMBER OF G-BANDED CELLS EXAMINED: 20-30.
  • TYPE OF BANDING: G-banding routinely used.
  • BANDING LEVEL: variable, depending on type of tumor.
  • KARYOTYPES: 20 G-banded cells completely analyzed. Two karyotypes.
FISH and Molecular Tests 
  • Fluorescence in situ hybridization (FISH) tests, including Telomere FISH and spectral karyotyping, are usually done in conjunction with Cytogenetic Analysis to further identify chromosomal rearrangements. However, in some cases (known deletion syndromes), a FISH only test may be done.
  • Interphase FISH testing is done for hematologic disorders in Bone Marrow, for bladder cancer recurrance in Urine and for aneuploidy in Amniotic Fluid.
  • PCR testing is done for the detection and characterization of Y chromosome disorders including the detection of Y chromosome deletions associated with infertility in males.
  • Array CGH testing for genome analysis to be offered Fall 2009.

Research Services

  • Karyotype and FISH analysis for established mouse and human cell lines are offered at reasonable rates for UW and outside researchers.
  • Call the lab for available tests and pricing: 206-598-4488. Our request form is available here.

  

Additional Information
For additional information regarding Cytogenetics services, CPT codes and pricing, please call (206) 598-4488. After hours,
weekends and holidays, our recorded message will instruct you to call the UWMC paging operator at (206) 598-6190
to have the Cytogenetics faculty member on-call paged. We are prepared to accept samples 24 hour/day, 7 days/week.