Flow Cytometry: Fresh Specimens

General Recommendations for the Handling of Fresh Specimens

Specimen Required

From resected specimen, sample a piece of the tumor (non-necrotic, trim away normal tissue). (A corresponding sample should be submitted for histologic diagnosis and designated as such in the gross dictation). Place sample in minimum essential media (MEM) plus 10% fetal calf serum and 10% DMSO. (If you are using your own transport media, which does not contain DMSO, do not freeze.)

Handling

It is important not to have the sample at room temperature. Exposure to room temperature can result in degradation, which can lead to false-positive aneuploid cell populations in the sample.

Labeling

The specimen label should include the patient name, identification number, and specimen source.

Shipping

  • Ship the same day on wet ice.
  • Ship overnight on dry ice.
  • A Phone call to notify laboratory of shipment is appreciated. Please do not ship for weekend or holiday delivery.

Pathology Service Request Form Required

DNA Flow Cytometry Service Request Form

Flow Cytometry DNA Analysis Report

Report contains analysis results including histogram, interpretation and supporting literature references.

Process Time

4 days from receipt.