Viable Hoechst 33342 Stain

  1. Cells should be maintained in the media that is normal for their growth.
  2. Add 10 µl of 1 mM Hoechst 33342/ml of media. Incubate for 30 minutes at 37°C. Optimal incubation time will vary with cell type.
  3. Cells must be maintained in Hoechst, DO NOT wash out the stain.
  4. Put the samples on ice after incubation in order to slow dye efflux.
  5. Run the samples on the flow cytometer as soon as possible (within minutes) of staining.

Hoechst 33342 (viable DNA stain) 1mM
For a 200 ml solution combine: * CALBIOCHEM cat.# 382065-Q
Bisbenzimide H 33342 Fluorochrome, Trihydrochloride.